Striated muscle of diaphragm
longitudinal and cross section
Ronald A. Bergman, Ph.D., Adel K. Afifi, M.D., Paul M. Heidger,
Peer Review Status: Externally Peer Reviewed
Rat, frozen section, osmium
tetroxide fixation and stain, 612 x.
Cells contain lipid droplets, neutral fats that are liquid at body temperature. Lipids are metabolized for energy or incorporated in cell membranes and other structures. In routine preparations, lipid is extracted and is not seen. In well-fixed tissues, clear round holes may sometimes be seen; these mark the site of lipid droplets within the cytoplasm of cells. The lipid in this preparation was fixed with osmium tetroxide, which renders it insoluble, and appears black. Compare with Plates 39 and 40.
Lipid droplets: Irregularly distributed within smaller muscle fibers. in contrast, larger fibers are devoid of lipid droplets. See Plates 70 and 72.
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