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Anatomy Atlases: Atlas of Microscopic Anatomy: Section 1 - Cells Atlas of Microscopic Anatomy: Section 5 - Muscular Tissue

Plate 5.66: Striated Muscle

Transverse striations
longitudinal section

Ronald A. Bergman, Ph.D., Adel K. Afifi, M.D., Paul M. Heidger, Jr., Ph.D.
Peer Review Status: Externally Peer Reviewed

Transverse striations
longitudinal section

Plate 5.66: Striated Muscle

Human, Helly's fluid, phosphotungstic acid
hematoxylin stain, A. 612 x; B. 1416 x.

Phosphotungstic acid hematoxylin is a stain particularly suited for the demonstration of striations in skeletal muscle. Iron hematoxylin and Mallory-azan are also effectively used for this purpose. Note that at low magnification only the two major cross striations can be seen. The dark band is the A band, and the light band is the I band. Higher magnifications are usually required to see the light-staining area in the center of the A band, which is known as the H zone, and the thin, dark line bisecting the I band, which is named the Z line. The repeating structural unit between two Z lines is called a sarcomere.

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