Ronald A. Bergman, Ph.D., Adel K. Afifi, M.D., Paul M. Heidger,
Peer Review Status: Externally Peer Reviewed
A. Sheep, Rossman's fixative, periodic acid-Schiff and
hematoxylin stains, 162 x.
B. Human, 10% formalin, H. & E., 162 x.
In this figure, the contrast between ordinary cardiac muscle fibers and their specialized variety, the Purkinje fibers, is evident. Purkinje fibers are larger than ordinary cardiac muscle fibers and stain less intensely. Note the clear areas in the cytoplasm of Purkinje fibers in B. These represent areas from which glycogen was lost during the preparation of the tissue. By contrast, in the Purkinje fibers seen in A, the glycogen is preserved by the fixation method used. Note the subendocardial location of Purkinje fibers.
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