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Anatomy Atlases: Atlas of Microscopic Anatomy: Section 1 - Cells Atlas of Microscopic Anatomy: Section 5 - Muscular Tissue

Plate 5.78: Purkinje Fibers

Ronald A. Bergman, Ph.D., Adel K. Afifi, M.D., Paul M. Heidger, Jr., Ph.D.
Peer Review Status: Externally Peer Reviewed


PURKINJE FIBERS

Plate 5.78: Purkinje Fibers

A. Sheep, Rossman's fixative, periodic acid-Schiff and hematoxylin stains, 162 x.
B. Human, 10% formalin, H. & E., 162 x.

In this figure, the contrast between ordinary cardiac muscle fibers and their specialized variety, the Purkinje fibers, is evident. Purkinje fibers are larger than ordinary cardiac muscle fibers and stain less intensely. Note the clear areas in the cytoplasm of Purkinje fibers in B. These represent areas from which glycogen was lost during the preparation of the tissue. By contrast, in the Purkinje fibers seen in A, the glycogen is preserved by the fixation method used. Note the subendocardial location of Purkinje fibers.

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