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Anatomy Atlases: Atlas of Microscopic Anatomy: Section 1 - Cells Atlas of Microscopic Anatomy: Section 1 - Cells

Plate 1.4: Cell Division

Lymph node

Ronald A. Bergman, Ph.D., Adel K. Afifi, M.D., Paul M. Heidger, Jr., Ph.D.
Peer Review Status: Externally Peer Reviewed


CELL DIVISION
Lymph node

Plate 1.4: Cell Division

 

Rat, Helly's* fluid, Mallory's stain, 1416 x.

 

This plate illustrates the nuclear events in mitosis. They will be described in the sequence in which they occur.

Interphase (A): Non-dividing or resting stage. The chromatin appears as an irregular reticular meshwork. The nuclear membrane, or envelope, and the nucleolus are distinctly seen. Chromosomes are not visible.

Early prophase (B): Nuclear membrane and nucleolus disappear. Granularity of the nucleus is markedly increased, and filamentous structures are seen. These granules and filaments represent the chromosomes, which become shorter and thicker in this stage.

Late prophase (C): The thread- or rod-like character of the chromosomes is more apparent. Each chromosome consists of two coiled chromatids, which are not visible in this preparation. The disappearance of the nuclear membrane allows mixing of nuclear and cytoplasmic material.

Metaphase (D): Chromosomes appear condensed and line up in the equatorial plane (metaphase plate) of the cell. Each chromosome is still composed of two paired chromatids.

Anaphase (E): The daughter chromosomes (chromatids) separate and are drawn to opposite poles of the cell. They remain separate and tightly coiled, and appear at this magnification to be fused. Cytoplasmic division begins.

Telophase (F): The two distinct groups of daughter chromosomes (chromatids) appear fused and tightly packed. Cytoplasmic division is completed. Nuclear membranes re-form and nucleoli reappear.

*Helly was a twentieth-century Swiss pathologist.

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